Vol.7 No.2 June 2002
| Volume 7 (2002) pp 195-202 | |
| Title | THE EFFECT OF PONERATOXIN ON NEUROMUSCULAR TRANSMISSION IN THE RAT DIAPHRAGM |
| Authors | Andrzej B. Hendrich1, Jerzy W. Mozrzymas1, Danuta Konopinska2 and Maria Scuka3 |
| Abstract | The effect of the ant venom neuropeptide - poneratoxin (PoTX) - on neuromuscular transmission in rat diaphragm tissue was studied by means of intracellular recordings of spontaneous miniature endplate potentials (MEPPs) and of nerve evoked endplate potentials (EPPs). A 2 mM concentration of PoTX caused a pronounced but transient increase in MEPPs frequency. Moreover, within the first few minutes of PoTX administration, the area, rise time and half decay time of MEPPs gradually decreased, reaching steady values after 15-20 min. The alteration of the area, rise time and half decay time of EPPs after PoTX application was similar to that observed for MEPPs. We conclude that PoTX affects neuromuscular transmission in rat tissue, and suggest that PoTX could exert both pre- and postsynaptic effects.. |
| Address and Contact Information | 1Department of Biophysics, Wrocław Medical University, T. Chałubinskiego 10,
50-368 Wrocław, Poland, 2Institute of Chemistry, University of Wrocław, P. Joliot-Curie 14, 50-383 Wrocław, Poland, 3Istituto di Fisiologia e Patologia, Universita degli Studi di Trieste, Via A.Fleming 22, IĹ 34127 Trieste, Italy |
 ![[Rozmiar: 1332 bajtĂłw]](pic/abstract.gif){kind=small} |
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| Volume 7 (2002)pp 203-211 | |
| Title | FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) AS A TOOL TO STUDY DNA CONDENSATION WITH HEXADECYLTRIMETHYLAMMONIUM BROMIDE (HTAB) |
| Authors | Teresa Kral1, 2*, Martin Hof2, Piotr Jurkiewicz3 and Marek Langner3,4 |
| Abstract | The presented data show that the FCS technique can be used to detect the DNA condensation process induced with the cationic compound hexadecyltrimethylammonium bromide (HTAB). We have shown that HTAB induces plasmid condensation upon interaction with it. Condensation can be considered to be complete when the diffusion constant reaches its maximum. The HTAB induced increase in diffusion time does not correlate well with the changes observed when count rate and particle number are considered. This observation contradicts data published for another cationic agent, spermine. This apparent discrepancy proves that the mechanisms of interaction between these compounds and DNA are different. Consequently, the different characters of the plots of count rate, diffusion time, and particle number versus condensing agent concentration can be a source of additional information about the nature of cationic compound-DNA interaction. |
| Address and Contact Information | 1Department of Physics and Biophysics, Agricultural University, Norwida 25,
50-354 Wrocław, Poland, 2J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic and Center for Complex Molecular Systems and Biomolecules, Dolejskova 3, CZ-18223 Prague, Czech Republic, 3Wrocław University of Technology, Institute of Physics, Wyb. Wyspiańskiego 27, 50-370, Wrocław, Poland, 4Academic Centre for Biotechnology of Lipid Aggregates, Przybyszewskiego 63/77, 51-148 Wrocław, Poland |
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W. MEJBAUM-KATZENELLENBOGEN'S MOLECULAR BIOLOGY SEMINARS
9. LIPOSOMES. FROM MODELS TO APPLICATIONS
MAY 26-29, 2002 WROCLAW/SZKLARSKA POREBA/PIECHOWICE, POLAND
| Volume 7 (2002) pp 213-306 | |
| Invited Lectures | |
| Lectures List |
USE OF THE POST-INSERTION METHOD FOR THE FORMATION OF LIGAND-COUPLED LIPOSOMES Theresa M. Allen, Puja Sapra and Elaine Moase - p217-219LIPID-BASED ANTIFUNGAL AGENTS Sevtap Arikan - p220-221ELASTIC VESICLES: INTERACTION WITH HUMAN SKIN AND DRUG TRANSPORT J.A. Bouwstra, A. De Graaff, W. Groenink and P.L. Honeywell - p222-223SELF-REGULATING `SMART CARRIERS´ FOR NON-INVASIVE AND TARGETED DRUG DELIVERY Gregor Cevc - p224-225STABILIZED PLASMID-LIPID PARTICLES FOR SYSTEMIC GENE THERAPY Pieter R. Cullis - p226RECONSTITUTION OF FLIPPASE ACTIVITY INTO LIPOSOMES Philippe F. Devaux - p227-229AN APPROXIMATE COOPERATIVITY ANALYSIS OF THE PHASE TRASITIONS OF DPPC-DOCNA DISPERSIONS Stefan Hobai and Zita Fazakas - p230ON THE MECHANISM OF PROTEIN-INDUCED MEMBRANE FUSION: FROM MODEL TO BIOLOGICAL MEMBRANES Dick Hoekstra, Isabelle Martin, Nicoletta Kahya, Jean-Marie Ruysschaert and Eve Pécheur - p231-232LIPOSOMES AS ULTRASOUND IMAGING CONTRAST AGENTS AND AS ULTRASOUND-SENSITIVE DRUG DELIVERY AGENTS Shaoling Huang, Andrew J. Hamilton, Susan D.Tiukinhoy, Ashwin Nagaraj, Bonnie J. Kane, Melvin Klegerman, David D. McPherson and Robert C. MacDonald - p233-235IN VITRO DELIVERY OF ANTISENSE OLIGONUCLEOTIDES Ilpo Jääskeläinen, Paavo Honkakoski and Arto Urtti - p236-237COMPUTATIONAL MODELING OF DNA-CATIONIC LIPID COMPLEXATION Mikko Karttunen, Antti L. Pakkanen, Paavo K.J. Kinnunen and Kimmo Kaski - p238-239MEMBRANES AT INTERFACES: STRUCTURE STUDIES BY AFM AND TIME-RESOLVED NEUTRON REFLECTIVITY Beate Klösgen, Th. Spangenberg, H. Niehus, T. Gutberlet, R. Steitz and G. Fragneto - p240LIPOSOMES - AN INVALUABLE TOOL IN BISPHOSPHONATE RESEARCH Jukka Mönkkönen - p241-242FORMATION AND CHARACTERISATION OF NON-TOXIC ANIONIC LIPOSOMES FOR DELIVERY OF THERAPEUTIC AGENTS TO THE PULMONARY AIRWAYS M. Reza Mozafari, Celia J. Reed, Christopher Rostron, Cengiz Kocum and Erhan Piskin - p243-244TOWARDS PROTO-CELLS : "PRIMITIVE" LIPID VESICLES ENCAPSULATING GIANT DNA AND ITS HISTONE COMPLEX Shin-Ichirou M. Nomura, Kanta Tsumoto, Kenichi Yoshikawa, Guy Ourisson and Yoichi Nakatani - p245-246CANCER ANTINEOVASCULAR THERAPY Naoto Oku, Tomohiro Asai, Kohta Kurohane, Koh Watanabe, Koichi Kuromi, Yukihiro Namba, Koichi Ogino and Takao Taki - p247-248BIOPHYSICAL AND TRANSFECTION PROPERTIES OF DNA-DIC14-AMIDINE COMPLEXES Jean-Marie Ruysschaert - p249-250TARGETING LIPOSOMES AND LIPOPLEXES TO CELLS IN THE LIVER Gerrit Scherphof, Gerben Koning, Martin Bartsch, Xuedong Yan and Jan Kamps - p251-254TARGETING OF LIPOSOMES TO ANGIOGENIC ENDOTHELIAL CELLS Raymond M. Schiffelers, Grietje Molema, Timo L.M. Ten Hagen, Adriënne P.C.A. Janssen, Astrid J. Schraa, Robbert J. Kok, Gerben A. Koning and Gert Storm - p255BLOOD FLOW ENHANCEMENT IN SKIN OR ORAL MUCOSA AFTER THE TOPICAL APPLICATION OF LIPOSOME ENTRAPPED RUBIFA-CIENT AS MEASURED BY EPR OXIMETRY IN VIVO: THE INFLUENCE OF SIZE AND LIPOSOME COMPOSITION Marjeta Šentjurc - p256-258SOLVENT RELAXATION IN PHOSPHOLIPID BILAYERS: PHYSICAL UNDERSTANDING AND BIOPHYSICAL APPLICATIONS Jan Sýkora and Martin Hof - p259-261POLYUNSATURATED LIPIDS IN MEMBRANE FUSION EVENTS Walter E. Teague, Nola L. Fuller, R. Peter Rand and Klaus Gawrisch - p262-264TAT PEPTIDE-MODIFIED LIPOSOMES FOR INTRACELLULAR DELIVERY OF DRUGS AND DNA Vladimir P. Torchilin - p265-267DEVELOPMENT OF LIPOSOMAL GENE TARGETING FOR THERAPEUTIC INTERVENTION OR PREVENTION OF RESTENOSIS Ewa Zant-Przeworska, Jan A.A.M. Kamps, Anton J.M. Roks, Wiek H. Van Gilst and Gerrit L. Scherphof - p268-269INHIBITION OF CARBOHYDRATE MEDIATED CELL ADHESION BY LIPOSOMES AS A POSSIBILE WAY TO PREVENT TUMOUR METASTASIS Reiner Zeisig, Renate Stahn and Iduna Fichtner - p270-271EVIDENCE FOR THE HYDROLYSIS OF TOPICAL APPLIED LIPOSOMAL LIPIDS IN HUMAN STRATUM CORNEUM Sebastian Zellmer - p272 |
| Oral and poster comunications | |
| List of Comm. |
IN VIVO BEHAVIOR AND DISPOSITION OF 3H-CHOLESTERYL ETHER LABELED LIPOSOMES FOLLOWING INTRAVENOUS ADMINISTRA-TION TO MICE: COMPARISON WITH AN INCORPORATED 14C-INULIN AS AN AQUEOUS PHASE MARKER Michael Anderson, Celine Paradis and Abdelwahab Omri - p275TUMOR LOCALIZATION OF TECHNETIUM-99m-ALPHA-D-GLUCOSE-1-PHOSPHATE USING DUAL RADIOLABELLED STEALTH LIPOSOME ENGINEERED WITH IOR CEA-1 MONOCLONAL ANTIBODIES Sandeep Kumar Bagga, Thakuri Singh, Anil Babbar, Uday Pratap Singh Chauhan, Sri Nath Sharma and Roop Kishen Khar - p276-277PROLIPOSOMES FOR ORAL DRUG ADMINISTRATION Guru V. Betageri and Milton B. Yatvin - p278THE OXIDATIVE ACTIVITY OF SOME ORGANOPHOSPHOROUS COMPOUNDS Dorota Bonarska, Halina Kleszczyńska and Janusz Sarapuk - p279THE KINETICS OF HEMOLYSIS AS AN INDICATOR OF SPHE-ROCYTOSIS Anna Chmura, Grzegorz Paździor and Marek Langner - p280LIPOSOME-ENCAPSULATED CARBAPENEMS Zuzanna Drulis-Kawa, Jerzy Gubernator, Kamil Kostyń, Włodzimierz Doroszkiewicz and Arkadiusz Kozubek - p281MEMBRANE-ACTIVE AMINOPHOSPHONATES Anna Dziamska and Janusz Sarapuk - p282THE STRUCTURE-PROPERTY RELATIONSHIPS OF FLAVONOID COMPOUNDS AS POTENTIAL DETOXICANTS Janina Gabrielska, Monika Soczyńska-Kordala and Stanisław Przestalski - p283BIOCHANIN A SIMILARLY INFLUENCES THE FLUIDITY OF LIPOSOMES FORMED FROM CHARGED AND ZWITTERIONIC LIPIDS Andrzej B. Hendrich, Jarosław Żugaj and Krystyna Michalak - p284PHARMACOKINETIC MODELING OF THE IN VIVO FATE OF LONG-CIRCULATING LIPOSOMES IN RATS Rafał Hrynyk, Josbert M. Metselaar, Gert Storm and Marek Langner - p285THE ACCELERATED CLEARANCE ON REPEATED INJECTION OF PEGYLATED LIPOSOMES IN RATS: LABORATORY AND HISTOPATHOLOGICAL STUDY Tatsuhiro Ishida, Ryuhei Maeda, Masako Ichihara, Yasuko Mukai, Yoko Motoki, Yoshie Manabe, Kenji Irimura and Hiroshi Kiwada - p286INTERACTION OF OLIGONUCLEOTIDES WITH POSITIVELY CHARGED LIPOSOMES USING FLUORESCENCE TECHNIQUES Piotr Jurkiewicz, Teresa Kral, Andrzej Okruszek, Martin Hof and Marek Langner - p287STUDIES ON INCORPORATING SMALL FLUORESCENT MOLECULES INTO PAMAM DENDRIMERS Barbara Klajnert and Maria Bryszewska - p288A DERIVATIVE OF 5-H-INDOLO[2,3-b]QUINOLINE - A NOVEL LIPOSOMALLY-FORMULATED ANTICANCER AGENT Anna Kociubińska, Jerzy Gubernator, Joanna Godlewska, Maria Stasiuk, Arkadiusz Kozubek, Wanda Peczyńska-Czoch, Adam Opolski and Łukasz Kaczmarek - p289THE ROLE OF THE LIPID POLAR PART IN RIPPLE PHASE FORMATION Krystian Kubica - p290LONG-CIRCULATING LIPOSOMES FOR I.V. TARGETED DELIVERY OF GLUCOCORTICOIDS IN ARTHRITIS Josbert M. Metselaar, Marca H. M. Wauben, Otto C. Boerman, Peter L. Van Lent And Gert Storm - p291-292LIPID MEMBRANE PERTURBATION CAUSED BY SOME ISOFLAVONES AND PHENOTHIAZINES, AND THE ACTIVITY OF THESE COMPOUNDS AS INHIBITORS OF MULTIDRUG RESISTANCE Krystyna Michalak , Andrzej B. Hendrich, Olga Wesołowska, Andrzej Poła, Barbara Łania-Pietrzak, Noboru Motohashi, Yoshiaki Shirataki and Joseph Molnar - p293THE ANTIPROMOTING EFFECT OF ALKYLOGLYCEROLS FROM SHARK LIVER OIL Joanna Miłoszewska and Marcin Krotkiewski - p294SPECTROPHOTOMETRIC DETERMINATION OF THE PHOSPHATI-DYLCHOLINE LIPOSOMES/WATER PARTITION COEFFICIENTS AND THE PROTONATION EQUILIBRIUM CONSTANTS OF PHE-NOTHIAZINE DERIVATIVES Andrzej Poła, Krystyna Michalak and Joseph Molnar - p295NON-GLYCEROL BASED LIPOFECTINS FOR GENE DELIVERY Nalam M Rao and Arabinda Chaudhuri - p296THE CONTRIBUTION OF PHOSPHATIDYLSERINE PARTITION AND TRANSMEMBRANE POTENTIAL TO THE INTERACTION OF MEROCYANINE 540 WITH LIPID BILAYERS Marcin Różalski, Iveta Waczulikova, Juraj Rievaj, Kristina Nagyova, Maria Bryszewska and Cezary Watala - p297MIKROCALORIMETRIC STUDIES ON THE INTERACTION OF N-DODECYL-N,N -DIMETHYL-N-BENZYLAMMONIUM HALIDES WITH PHOSPHATIDYLCHOLINE BILAYERS: A COUNTERION EFFECT Bożenna Różycka-Roszak, Adriana Przyczyna and Romuald Żyłka - p298THE ROLE OF MITOTIC VESICLES IN THE NUCLEAR ASSEMBLY Ryszard Rzepecki, Carl Smythe and Christopher J. Hutchison - p299PHOSPHOLIPID MONOLAYER AND LIPOSOME APPLICATION IN THE DETERMINATION OF THE PHOTOPHYSICAL CHARACTE-RISTICS OF MC 540 Libuša Sikurova - p300INTENSIVE TUMOR SUPPRESSION BY ANTI-ANGIOGENIC PHOTODYNAMIC THERAPY WITH POLYCATION-MODIFIED LIPOSOMAL PHOTOSENSITIZER Yoshito Takeuchi, Kohta Kurohane, Mamoru Nango and Naoto Oku - p301CHLOROPHYLL a IN LIPOSOMES - SPECTRAL AND ELECTROCHE-MICAL STUDIES Laura Tugulea and Ioan Iacovache - p302A NEW PHENOTHIAZINE DERIVATIVE ALTERS THE FLUIDITY AND THERMOTROPIC BEHAVIOUR OF PHOSPHATIDYLCHOLINE MODEL MEMBRANES Olga Wesołowska, Andrzej B. Hendrich, Noboru Motohashi and Krystyna Michalak - p303A LIPOSOME MODEL TO TREAT PSEUDOMONAS IN LUNGS OF CYSTIC FIBROSIS AND IMMUNE COMPROMISED PATIENTS Milton B. Yatvin - p304LIPOSOMES WITH A DOUBLE BILAYER - A STUDY ON STABILITY Zygmunt Zawada, Anna Michnik and Zofia Drzazga - p305
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THE INTERNATIONAL CONFERENCE ON LIFE SCIENCES 2001
Part II
September 22.-26. 2001, Gozd Martuljek, SLOVENIA
| Volume 7 (2002) pp 307 | |
| Invited Lectures | |
| Abstracts List |
HIP BIOMECHANICS IN ORTHOPAEDIC CLINICAL PRACTICE Vane Antolič, Veronika Kralj-Iglič, Aleš Iglič and Borut Pompe - p311-315IONIC MECHANISMS INVOLVED IN THE NODAL SWELLING OF MYELINATED AXONS CAUSED BY MARINE TOXINS Evelyne Benoit, Cesar Mattei, Gilles Ouanounou, Frederic A. Meunier, Dusan Suput, Frederic Le Gall, Michel Marquais, Marie Y. Dechraoui and Jordi Molgo - p317-321REQUIREMENTS FOR THE LOCALIZATION OF p130 Cas TO Z-LINES IN CARDIAC MYOCYTES Branka Kovačič-Milivojević, Caroline C. Damsky, David G. Gardner and Duško Ilić - p323-329A PARADIGM FOR QUANTIFYING VENTRICULAR CONTRACTION Joseph L.Palladino and Abraham Noordergraaf - p331-335A DOSIMETRIC METHOD OF TOTAL BODY IRRADIATION Mladen Vrtar - p337-340 |
| Communications | |
| List of Comm. |
CELL MARKERS IN THE RECOGNITION OF ACUTE MYELO-BLASTIC LEUKAEMIA SUBTYPES Dušan Andoljšek, Irena Preložnik Zupan, Darja Žontar, Peter Černelč, Uroš Mlakar, Mojca Modic, Jože Pretnar and Samo Zver - p343-345MODELLING THE STATE OF BONE CYSTS BY STATISTICAL ANA-LYSIS OF INTENSITIES OF X-RAY IMAGES Janez Brecelj and Velimir Bole - p347-349EQUINATOXIN II-INDUCED LYSIS OF THE CULTURED ENDOTHE-LIAL CELL LINE ECV-304 Matjaž Bunc, Janez Rozman, Radovan Starc, Peter Maček and Dušan Šuput - p351-353NICARDIPINE DIMINISHED EQUINATOXIN II-INDUCED DECREASE OF CORONARY FLOW IN ISOLATED RAT AND PIG HEARTS Matjaž Bunc, Janez Rozman, Alenka Vidmar, Peter Rakovec, Gorazd Drevenšek, Metka V. Budihna and Dušan Šuput - p355-357THE IMPORTANCE OF HLA DQB1*0602 TYPING IN SLOVENE PATIENTS WITH NARCOLEPSY Leja Dolenc-Grošel and David B. Vodušek - p359-360THE OUTCOME OF AUTOLOGOUS CHONDROCYTE TRANSPLAN-TATION TREATMENT OF CARTILAGE LESIONS IN THE KNEE Matej Drobnič, Nevenka Kregar-Velikonja, Damjan Radosavljevič, Miro Gorenšek, Branko Koritnik, Elvira Maličev, Gordana Wozniak, Matjaž Jeras and Miomir Kneževič - p361-363BIOMECHANICAL ANALYSIS OF CHIARI OSTEOTOMY Simon Herman, Veronika Kralj-Iglič, Aleš Iglič, Vane Antolič and Srečko Herman - p365-366THE MUSCLE ADAPTATION PROCESS AS A RESULT OF PATHOLOGICAL CHANGES OR SPECIFIC TRAINING PROCEDURES Katarina Kerševan, Vojko Valenčič, Srdjan Djordjević and Boštjan Šimunič - p367-369EHOCARDIOGRAPHIC EVALUATION OF REGIONAL LEFT ATRIAL FUNCTION AFTER RAPID ATRIAL PACING Mirta Koželj, Igor Zupan, Janos Butinar, Alenka Seliškar and Peter Rakovec - p371-373DIFFERENCES IN THE OXYGENATION OF THE FOREARM MUSCLE DURING ISOMETRIC CONTRACTION IN TRAINED AND UNTRAINED SUBJECTS Anton Usaj - p375-377THE EFFECT OF PROPRIOCEPTIVE TRAINING IN PATIENTS WITH RECURRENT DISLOCATION OF THE PATELLA Rok Vengust, Vojko Strojnik, Vinko Pavlovčič, Vane Antolič and Oskar Zupanc - p379-380HAZARD IDENTIFICATION FOR 3,5-DICHLOROPHENOL IN THE AQUATIC ENVIRONMENT Jana Zagorc-Končan, Andreja Žgajnar Gotvajn and Tatjana Tišler - p381-382IMPAIRED SINUS NODE FUNCTION AND GLOBAL ATRIAL CONDUCTION TIME AFTER HIGH RATE ATRIAL PACING IN DOGS Igor Zupan, Mirta Koželj, Janos Butinar and Peter Rakovec - p383-384 |