Vol. 3 No. 2 June 1998
| Volume 3 (1998) pp 103-109 | |
| Title | DOES THE APOPTOTIC FRAGMENTATION OF DNA STARTS BY EXCISION OF CHROMOSOMAL DNA LOOP DOMAINS? |
| Authors | E. Ioudinkova, A. Bystritsky and S. Razin |
| Abstract | In this paper we are presenting a critical review of works demonstrating that at the early stages of apoptosis eukaryotic DNA is cleaved to large fragments the length of which is several tens of thousands of nucleotide pairs. Evidence is presented that this fragmentation proceeds in a specific fashion and that the character of the fragmentation reflects the general principle of eukaryotic genome organization into structural-functional domains. |
| Address and Contact Information | Institute of Gene Biology, 34/5 Vavilov St., 117334, Moscow, Russia |
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| Volume 3 (1998) pp 111-117 | |
| Title | SIMPLIFIED SOLUTION OF LACTATE DEHYDROGENASE CATALYSED REACTION |
| Authors | M. Kuczek |
| Abstract | The lactate dehydrogenase catalysed reaction shows lag phase. This lag phase is easy for explanation if the consecution of first and second order equilibrium reactions were assumed for calculation of pyruvate trace concentration. The same explanation was accepted for calculation of significant pyruvate concentration. For calculation significant pyruvate concentration the consecution of simple Michaelis-Menten type and second order reactions was assumed. The exact solution of reaction rate equations system for this complex reaction was counted. |
| Address and Contact Information | Institute of Chemistry, University of Opole Oleska 48, 45-052 Opole, Poland |
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| Volume 3 (1998) pp 119-132 | |
| Title | SYNTHESIS OF AMINOPHOSPHOLIPIDS IN Saccharomyces cerevisiae AND CHINESE HAMSTER OVARY CELLS. FROM MUTAGENESIS TO GENES AND CELLULAR FUNCTION |
| Authors | J. Lenart and S. Pikula* |
| Abstract | The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes. |
| Address and Contact Information | Laboratory of Lipid Biochemistry, Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland * Corresponding author. Fax: (+4822) 822 5342; E-mail: slawek@nencki.gov.pl |
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| Volume 3 (1998) pp 133-143 | |
| Title | THE EFFECT OF POLYETHYLENE GLYCOL ON SOME PROPERTIES OF ERYTHROCYTE MEMBRANE |
| Authors | M. Danieluk 1,2 H. Hagerstrand3, A. Jezierski 4, J. Jezierska4 and A. F. Sikorski1 |
| Abstract | Polyethylene glycol (PEG) induces fusion of cells creating fused cell pairs and larger cell aggregates. However the precise mechanism of its action on cell membranes remains unclear. In the present study we attempted to determine how PEG interacts with the membrane of red blood cell. It is known that PEG, at concentrations that evoke cell fusion, induces strong swelling of erythrocytes which in appeared to be insensitive to elevated ionic strength of the solution. This swelling, as well as fusion rate, is independent of the initial shape of erythrocytes induced by various amphiphiles. PEG in the concentrations used usually as a fusogen evoked haemolysis (up to 50%). Again, this effect was not inhibited by elevated ionic strength of the buffer. Further experiments revealed that PEG changes the membrane properties such as surface pressure of lipid monolayer prepared from total erythrocyte lipids and mobility of acyl hydrocarbon chains of membrane lipids as measured using 5-doxyl stearate as a spin probe. |
| Address and Contact Information | 1University of Wroclaw, Institute of Biochemistry, Laboratory of Cell Biology, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland, 2Nencki Institute of Experimental Biology, Department of Cellular Biochemistry, Pasteura 3, 02093 Warsaw, Poland, 3Abo Akademi University, Department of Biology, Biocity 20520 Abo/Turku, Finland, 4University of Wrocław, Institute of Chemistry, Joliot-Curie 14, 50383 Wrocław, Poland |
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| Volume 3 (1998) pp 145-150 | |
| Title | TOROCYTES - A NEW CLASS OF VESICLE SHAPES |
| Authors | H. Hagerstrand1, V. Kralj-Iglic2, M. Bobrowska-Hagerstrand2, K. Bialkowska3, B. Isomaa1 and A. Iglic4 |
| Abstract | Torocyte shaped endovesicles with a low relative volume were induced in human erythrocytes by polyethyleneglycol dodecylether (C12E8). It is suggested that the torocyte endovesicles are formed in a process where an initially stomatocytic invagination loses volume while maintaining its large surface area. The boundaries of the phase diagram of the observed torocytes are described. |
| Address and Contact Information | 1Department of Biology, Abo Akademi University, FIN-20520, Abo/Turku, Finland, 2Institute of Biophsics, Medical Faculty, University of Ljubljana, SI-1000, Ljubljana,Slovenia, 3Institute of Biochemistry, University of Wroclaw, PL-51148, Wroclaw, Poland and 4Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, SI-1000, Ljubljana, Slovenia |
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| ERRATUM to the article published in Cellular & Molecular Biology Letters volume 3, number 2, 1998, pp 145-150 | |
| Article | TOROCYTES - A NEW CLASS OF VESICLE SHAPES |
| Authors | H. Hagerstrand1, V. Kralj-Iglic2, M. Bobrowska-Hagerstrand2, K. Bialkowska3, B. Isomaa1 and A. Iglic4 |
| Abstract |
The formula (3) on page 147 should read: C2 = ± y"(1 + y'2)-3/2
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| Volume 3 (1998) pp 151-221 | |
| Seminar Title | W. MEJBAUM-KATZENELLENBOGEN'S SEMINARS ON MOLECULAR BIOLOGY. 5. MEMBRANE SKELETON. REGULATORY FUNCTION IN NORMAL AND ABNORMAL CELLS |
| Abstracts List |
CALCIUM IN SPECTRIN FUNCTION L. Backman, S. Lundberg, A. Buevich , I. Sethson and U. Edlund - p.152 BETAII SPECTRINS AND FODAXINS: INTERACTING PROTEINS IN MANY TISSUES A. J. Baines, V. Ohanian, E. Heerkens, G. Phillips and N. V. L. Hayes - p.153 REGULATION OF ANNEXIN VI FUNCTION BY ATP J. Bandorowicz-Pikula, M. Danieluk, A. Wrzosek and S. Pikula - p.155 A SPECTRIN MEMBRANE SKELETON OF THE GOLGI COMPLEX K. A. Beck - p.157 MOLECULAR PHYSIOLOGY OF THE ANKYRIN FAMILY: LESSONS FROM DISRUPTION OF ANKYRIN B AND ANKYRING GENES IN MICE V. Bennett, D. Zhou, S. Tuvia, S. Lambert and - p.158 IDENTIFICATION OF A NOVEL, PROLINE-RICH PROTEIN WHICH INTERACTS WITH a-SPECTRIN SH3 DOMAIN K. Bialkowska, V-P. Lehto and J.E.B Fox - p.159 MOLECULAR BASIS OF RESISTANCE OF WINTER TRITICALE TO APHIDS A. Piotr Ciepiela and M. Lubkowski - p.161 HEREDITARY DEHYDRATED STOMATOCYTOSIS : RECENT ADVANCES AND QUESTIONS J. Delaunay, S. Grootenboer, O. Schischmanoff, T. Cynober, G. Tchernia, J.P.Dommergues, M. Bost, G. Stewart, S. Perrotta, M. Carella, - p.161 CRYSTAL STRUCTURE F-ACTIN BINDING DOMAIN FROM HUMAN b-SPECTRIN K. Djinovic Carugo, S. Banuelos and M. Saraste - p.163 HEREDITARY ELLIPTOCYTOSIS: INFLUENCE OF TRANS-ACTING LOW EXPRESSION ALLELES ON DISEASE SEVERITY S.W. Eber, D.C. Boemmer and S. Ullmann - p.164 REGULATION OF EXOCYTOSIS BY CYTOSKELETON IN NEUROENDOCRINE CELLS S. Gasman, S. Chasserot-Golaz, M.F. Bader and D. Aunis - p.166 ANNEXINS AND MEMBRANE DYNAMICS V. Gerke - p.167 THE SPECTRIN MEMBRANE SKELETON: A KEY ELEMENT IN SICKLE CELL DISEASE S. R. Goodman - p.168 STRUCTURAL STUDIES OF CLONED SEGMENTS OF CHICKEN BRAIN a-SPECTRIN V.L. Grum, D. Li, R.I. MacDonald, A. Mondragon and D.P. Pantazatos - p.169 THE LAMPREY (Lampetra fluviatilis) ERYTHROCYTE; MORPHOLOGY, ULTRASTRUCTURE, MAJOR PLASMA MEMBRANE PROTEINS AND PHOSPHOLIPIDS, AND CYTOSKELETAL ORGANIZATION H. Hagerstrand, M. Danieluk and M. Nikinmaa - p.170 SEQUENCES HOMOLOGOUS TO ALPHA-SPECTRIN ARE EXPRESSED IN PLANTS B. Hanus-Lorenz, A. Hryniewicz, M. Lorenz - p.171 THE STRUCTURE OF THE RESTING AND ACTIVATED PLATELET CYTOSKELETON J. H. Hartwig, K. Barkalow, A. Azim, G. Bokoch, Y. Ohta, S. Nakamura, T. Stossel, K. Tolias and C. Carpenter - p.172 THE L1 FAMILY OF NEURAL CELL ADHESION MOLECULES - LINKING CELL ADHESION TO MEMBRANE SKELETON ORGANIZATION M. Hortsch and R.R. Dubreuil - p.174 SPICULATED RED BLOOD CELLS INDUCED BY INTERCALATION OF AMPHIPHILES A. Iglic , V. Kralj-Iglic and H. Hagerstrand - p.176 RATE CONSTANTS FOR REVERSIBLE DIDS BINDING TO BAND 3 PROTEIN IN HUMAN ERYTHROCYTE MEMBRANES T. Janas and T. Janas - p.177 DECREASE OF PKCa TRANSLOCATION TO THE ERYTHROCYTE MEMBRANE IN PATIENTS WITH CHRONIC RENAL FAILURE (CRF) J. Kaczmarek, G. Komorowska and M. Rybczynska - p.178 THE LYTIC ACTION OF POLYENE ANTIBIOTIC AMPHOTERICIN B ON HUMAN ERYTHROCYTES A. Knopik-Skrocka and J. Bielawski - p.179 ACTIN-BINDING MEMBRANE PROTEINS IN MOTILE CELLS E. J. Luna, R. K. Pope, K. N. Pestonjamasp and J. D.Wulfkuhle - p.180 EXPRESSION AND FUNCTION OF RED CELL PROTEIN 4.1 AND ITS HOMOLOGUES IN ERYTHROID AND NON-ERYTHROID CELLS N. Mohandas, M. Parra, - p.181 SPECTROSCOPIC PROPERTIES OF SELECTED FLUORESCENCE PROBES USED FOR LABELLING OF LYMPHOCYTE OR BLAST CELL MEMBRANES AND PLASMA HIGH DENSITY LIPOPROTEINS K. Michalak, A.Sidorowicz, M. Kuliszkiewicz-Janus, T. Modrzycka and A. Pola - p.182 PARTICIPATION OF INTEGRIN RECEPTORS IN SIGNAL TRANSDUCTION OF CONTACT INHIBITED C3H10T1/2 CELLS J. Miloszewska and - p.184 APC GENE IS MUTATED IN ENDOMETRIAL CANCER OF WOMEN R. Miturski, D. Burnouf, M. Nothisen, J. Tomaszewski, J. Jakowicki and R. Fuchs - p.185 THE EFFECT OF SODIUM TAURODEOXYCHOLATE ON HUMAN ERYTHROCYTES L. Mrowczynska and J. Bielawski - p.186 INFLUENCE OF SOME POLYOLS ON ERYTHROCYTE CYTOSKELETON PROTEINS O.A. Nardid, T.S. Dyubko, A.S. Soloviova and A.V. Zinchenko - p.187 TISSUE-DISTRIBUTION OF TWO a FODRIN ISOFORMS : CHARACTERIZATION AND STUDY OF THEIR SENSITIVITY TO CALPAIN G. Nicolas, L. Malbert-Colas, H. Gautero, C. Fournier and M.C. Lecomte - p.189 STUDY OF DYNAMICS BEE VENOM - INDUCED SHRINKAGE OF ERYTHROCYTES OF THE PATIENTS WITH ANEMIAS OF VARIOUS ETIOLOGY E.E. Nipot , S.B. Dyryavy and V.V. Cherepanov - p.191 MODULATED HUMAN GLOBIN GENE EXPRESSION: ROLE FOR ANTISENSE EXPRESSION VECTORS B. S. Pace - p.192 MECHANICAL STRESS AND HEMIN PRETREATMENT EFFECTS ON KINETIC PARAMETERS OF ERYTHROCYTES RESPONSE TO HYPEROSMOTIC SHOCK E.R. Pantaler and N.M. Shpakova - p.193 DETERMINATION OF THE CONTRACTILE MEMBRANE COMPLEX RESPONSIBLE FOR BEE VENOM-INDUCED SHRINKAGE OF THE ERYTHROCYTE MEMBRANE T.A. Parchenko - p.194 ACTOMYOSIN MOTOR IN THE MEROZOITE OF THE MALARIA PARASITE, Plasmodium falciparum: IMPLICATIONS FOR RED CELL INVASION J. C. Pinder - p.195 ERYTHROCYTE MEMBRANE SKELETON: A PUTATIVE PARTICIPATOR OF ADAPTIVE CELLULAR RESPONSE TO TEMPERATURE VARIATION S.V. Repina and N.V. Repin - p.196 TECHNIQUE TO MONITOR MULTIPLE SHAPE TRANSFORMATION EVENTS CAUSED BY THE INTERACTION OF RED BLOOD CELLS WITH BIOLOGICALLY ACTIVE SUBSTANCES S.V. Rudenko - p.198 THE ARRANGEMENT OF CORTICAL MICROTUBULES IN PLANT EPIDERMIS IS SENSITIVE TO THE CHANGES IN CELL WALL STRESSES T. Rusin , A. Szeja and Z. Hejnowicz - p.200 NO INCREASE OF PHOSPHATIDYLSERINE EXPOSURE ACCOMPANYING THE INCREASED MEMBRANE FLUIDITY IN ERYTHROCYTES OF ATAXIA TELANGIECTASIA GENE (ATM) CARRIERS M. Rybczynska, J. Kaczmarek and A. L. Pawlak - p.201 EXPRESSION OF c-erbB-2 ONCOPROTEIN IN PROLIFERATIVE AND HYPERPLASTIC HUMAN ENDOMETRIUM A. Semczuk, R. Miturski, J. Tomaszewski, K. Postawski, J. Kotarski and J. Jakowicki - p.202 NEWER FACETS OF SICKLE CELL ADHESIVENESS S. B. Shohet, and B. J.-M. Thevenin - p.203 INTERACTIONS OF SPECTRINS WITH PHOSPHOLIPIDS: POSSIBLE PHYSIOLOGICAL ROLE A. F. Sikorski - p.205 STOMATOCYTOSIS AND STOMATIN G. W. Stewart and S.E. Coles - p.206 CYTOSKELETON - BILAYER INTERACTION AND RED BLOOD CELL SHAPES S. Svetina - p.208 CONGENITAL DYSERYTHROPOIETIC ANAEMIA TYPE III A. Wahlin and H. Sandstrom - p.209 CASPASE AND CALPAIN MEDIATED PROTEOLYSIS OF NONERYTHROID SPECTRIN SUBUNITS IN NEURONAL CELLS UNDERGOING APOPTOSIS K. K.W. Wang, N. Rathna, - p.210 NEW INFORMATION ON CONGENITAL DYSERYTHROPOIETIC ANAEMIA, TYPE I S. N. Wickramasinghe - p.211 REGULATION OF SIGNAL TRANSDUCTION ACROSS SKELETON-ASSOCIATED ADHESION RECEPTORS IN PLATELETS J. E.B. Fox and S. Kulkarni not published in issue FAP52 AND EAST , TWO NEW FOCAL ADHESION PROTEINS O. Lohi, J. Meriläinen and V.-P. Lehto not published in issue |
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